NKX2-5eGFPw hESCs for isolation of human cardiac progenitors and cardiomyocytes

作者：Elliott David A; Braam Stefan R; Koutsis Katerina; Ng Elizabeth S; Jenny Robert; Lagerqvist Ebba L; Biben Christine; Hatzistavrou Tanya; Hirst Claire E; Yu Qing C; Skelton Rhys J P; Oostwaard Dorien Ward van; Lim Sue Mei; Khammy Ouda; Li Xueling; Hawes Susan M; Davis Richard P; Goulburn Adam L; Passier Robert; Prall Owen W J; Haynes John M; Pouton Colin W; Kaye David M; Mummery Christine L; Elefanty Andrew G; Stanley Edouard G^*

来源：Nature Methods, 2011, 8(12): 1037-+.

DOI：10.1038/NMETH.1740

NKX2-5 is expressed in the heart throughout life. We targeted eGFP sequences to the NKX2-5 locus of human embryonic stem cells (hESCs); NKX2-5(eGFP/w) hESCs facilitate quantification of cardiac differentiation, purification of hESC-derived committed cardiac progenitor cells (hESC-CPCs) and cardiomyocytes (hESC-CMs) and the standardization of differentiation protocols. We used NKX2-5 eGFP(+) cells to identify VCAM1 and SIRPA as cell-surface markers expressed in cardiac lineages.

• 出版日期2011-12