Deglycosylation of mAb by EndoS for Improved Molecular Imaging

作者：Gao Peng; Pinkston Kenneth L; Wilganowski Nathaniel; Robinson Holly; Azhdarinia Ali; Zhu Banghe; Sevick Muraca Eva M; Harvey Barrett R^*

来源：Molecular Imaging and Biology, 2015, 17(2): 195-203.

DOI：10.1007/s11307-014-0781-9

• mAb imaging agents
• Deglycosylation
• Near-infrared imaging
• EpCAM

Monoclonal antibodies (mAbs) have been shown preclinically as reliable targeting moieties for antigen imaging using near-infrared fluorescence (NIRF) molecular imaging. However, crystallizable fragment-gamma receptor (Fc gamma Rs) expressed on immune cells also bind mAbs through defined epitopes on the constant fragment (Fc) of IgG. Herein, we evaluate the potential impact Fc interactions have on mAb agent imaging specificity.
Through the removal of conserved glycans within the Fc domain, shown to have Fc/Fc gamma R interactions, we evaluate their impact on non-specific binding/accumulation of a NIRF-labeled mAb-based imaging agent in lymph nodes (LNs) in inflamed animals and in an orthotopic prostate cancer animal model of LN metastasis.
Deglycosylation of a murine mAb against the human epithelial cell adhesion marker using endoglycosidase EndoS significantly reduced non-specific binding in the LNs of inflamed animals and in cancer-negative LNs of tumor-bearing animals. Sensitivity remained unchanged while improvement in imaging specificity increased imaging accuracy.
The reduction of non-specific binding through deglycosylation of a mAb-based imaging agent shows that reducing Fc/Fc gamma R interactions can improve imaging accuracy.

• 出版日期2015-4