科研之友
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摘要
The purpose of the current study is to make a stability work about some liver's function parameters such as alanine transaminase (ALT; EC:2.6.1.2), L-lactate dehydrogenase (LDH; EC:1.1.1.27) and.-glutamyltransferase (GGT; EC: 2.3.2.2.) enzymes that are present in five control sera belonging to different brands and suppliers. The stability of enzymes stored for a specified period at 4 degrees C, 25 degrees C and -30 degrees C storage conditions, their activities were measured in autoanalyser accordance with the manufacturer's instructions for use in the commercial kit and periodic intervals. The control sera stored at 4 degrees C, belonging to one brand, the GGT activity decreased at the rate of 21.78% in 72 h. Similarly, the control serum stored at 25 degrees C, belonging to one brand, concerning the same parameters, the activity decreased at the rate of 19.49% as well. The control sera stored at 4 degrees C, belonging to two brands, the LDH activities, decreased at the rate of 28.04% and 42.17% in 72 h. Similarly, the control sera stored at 25 degrees C, belonging to three brands, concerning the same parameters, the activities decreased at the rate of 40%, 25.36% and 40.56% in 72 h. The stabilities of liver's parameters in control sera, stored at -30 degrees C for 96 hour and at 4 degrees C for 72 hour were satisfactory, generally. On the other hand, the stabilities of liver's parameters in control sera, stored at 25 degrees C for 72 h were unsatisfactory in large numbers.
- 出版日期2011
