Development of Multilayered Cell-Hydrogel Composites Using an Acoustic Focusing Technique

作者：Mazzoccoli Jason P; Feke Donald L^*; Baskaran Harihara; Pintauro Peter N

来源：Biotechnology Progress, 2010, 26(2): 600-605.

DOI：10.1002/btpr.332

• tissue engineering
• biomaterials
• bioengineering

Multi layered composites, composed of mammalian cells arranged in a hydro gel, have been prepared using an acoustic focusing technique. Acoustic focusing is a simple, nonchemical technique that allows for the fast arrangement of cells in matrices where the control of cell geometry is beneficial. Breast cancer cells (MDA-MB231) were dispersed in a 30 wt % solution of poly(ethylene glycol) diacrylate (PEGDA) of molecular weight 400 at a density of 5 x 10(6) cells/mL of PEGDA solution. An ultrasonic field was used to organize the cells before polymerization of PEGDA. Disk-shaped hydro gel composites, typically 1 cm in diameter and 2-mm thick were prepared based on a PEGDA solution volume of 130 mu L. At an acoustic frequency of 2.32 MHz, composites having cells positioned within concentric cylindrical shells interspersed with zones of cell-free hydro gel were produced. The cells were located in annuli approximately 80-mu m thick and about 300 pm apart. The structure and viability of the cells within these constructs were studied using a fluorescent LIVE/DEAD assay. The viability of the cells was on the order of 50%. For the conditions used in this study, cell death was primarily attributed to exposure of cells to the PEGDA solution prior to polymerization, rather than adverse effects of polymerization or the sound field itself.

• 出版日期2010-4