The identification and functional annotation of RNA structures conserved in vertebrates

作者：Seemann Stefan E; Mirza Aashiq H; Hansen Claus; Bang Berthelsen Claus H; Garde Christian; Christensen Dalsgaard Mikkel; Torarinsson Elfar; Yao Zizhen; Workman Christopher T; Pociot Flemming; Nielsen Henrik; Tommerup Niels; Ruzzo Walter L; Gorodkin Jan^*

来源：Genome Research, 2017, 27(8): 1371-1383.

DOI：10.1101/gr.208652.116

Structured elements of RNA molecules are essential in, e.g., RNA stabilization, localization, and protein interaction, and their conservation across species suggests a common functional role. We computationally screened vertebrate genomes for conserved RNA structures (CRSs), leveraging structure-based, rather than sequence-based, alignments. After careful correction for sequence identity and GC content, we predict similar to 516,000 human genomic regions containing CRSs. We find that a substantial fraction of human-mouse CRS regions (1) colocalize consistently with binding sites of the same RNA binding proteins (RBPs) or (2) are transcribed in corresponding tissues. Additionally, a CaptureSeq experiment revealed expression of many of our CRS regions in human fetal brain, including 662 novel ones. For selected human and mouse candidate pairs, qRT-PCR and in vitro RNA structure probing supported both shared expression and shared structure despite low abundance and low sequence identity. About 30,000 CRS regions are located near coding or long noncoding RNA genes or within enhancers. Structured (CRS overlapping) enhancer RNAs and extended 3' ends have significantly increased expression levels over their nonstructured counterparts. Our findings of transcribed uncharacterized regulatory regions that contain CRSs support their RNA-mediated functionality.

• 出版日期2017-8