科研之友
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摘要
This study was designed to determine the nutritional lipid requirement of Manchurian trout and to investigate the effects of lipid concentrations on the antioxidant status in larvae with experimental diets with different lipid levels. Oxidative stress differences between different organs and tissues were also assessed. Manchurian trout larvae were fed for 35 days and, during that period, growth and survival, the activity of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPX) and content of malondialdehyde (MDA) in viscera, muscle, gill and brain of four diets, lipid levels from 15 to 30%, and control treatment were measured. Growth rates were similar, but survival was low, between high and low dietary lipid levels. SOD activity was stimulated in viscera, muscle and brain in high lipid diets, but reduced in gills with increased lipid content. SOD was kept lower in the control group. GPX activity was inhibited in viscera and stimulated in gill, muscle and brain. CAT activities were enhanced by all treatments and showed the lowest values in the control. Lipid peroxidation of the diet was promoted in all organs, excluding the gill which showed no regular pattern. MDA content increased with increased dietary lipid levels in viscera, muscle and brain. Our results indicate that the most appropriate lipid requirement is probably 20-25% and a higher dietary level of lipids might induce oxidative stress in Manchurian trout larvae. The brain and gill were probably the most sensitive organs to oxidative damage.
- 出版日期2009-7
- 单位东北农业大学
