A Role of Oral Bacteria in Bisphosphonate-induced Osteonecrosis of the Jaw

作者：Mawardi H; Giro G; Kajiya M; Ohta K; Almazrooa S; Alshwaimi E; Woo S B; Nishimura I; Kawai T^*

来源：Journal of Dental Research, 2011, 90(11): 1339-1345.

DOI：10.1177/0022034511420430

• bisphosphonate-induced osteonecrosis of the jaw
• pamidronate
• gingival fibroblast
• KGF
• wound healing
• Fusobacterium nucleatum

No consensus has yet been reached to associate oral bacteria conclusively with the etio-pathogenesis of bisphosphonate-induced osteonecrosis of the jaw (BONJ). Therefore, the present study examined the effects of oral bacteria on the development of BONJ-like lesions in a mouse model. In the pamidronate (Pam)-treated mice, but not control non-drug-treated mice, tooth extraction followed by oral infection with Fusobacterium nucleatum caused BONJ-like lesions and delayed epithelial healing, both of which were completely suppressed by a broad-spectrum antibiotic cocktail. Furthermore, in both in vitro and in vivo experiments, the combination of Pam and Fusobacterium nucleatum caused the death of gingival fibroblasts (GFs) and down-regulated their production of keratinocyte growth factor (KGF), which induces epithelial cell growth and migration. Therefore, in periodontal tissues pre-exposed to bisphosphonate, bacterial infection at tooth extraction sites caused diminished KGF expression in GFs, leading to a delay in the epithelial wound-healing process that was mitigated by antibiotics.

• 出版日期2011-11