A sindbis virus replicon-based DNA vaccine encoding the rabies virus glycoprotein elicits immune responses and complete protection in mice from lethal challenge

作者：Saxena Sonal; Dahiya Shyam S; Sonwane Arvind A; Patel Chhabi Lal; Saini Mohini; Rai A; Gupta Praveen K^*

来源：Vaccine, 2008, 26(51): 6592-6601.

DOI：10.1016/j.vaccine.2008.09.055

• DNA vaccine
• Rabies
• Sindbis
• Replicase
• Immunization
• Mice

A sindbis virus replicon-based DNA vaccine encoding rabies virus glycoprotein (G) was developed by sub-cloning rabies G gene into a sindbis virus replicon-based vaccine vector (pAlpha). The self-amplification of RNA transcripts and translation efficiency of rabies G was analyzed in pAlpha-Rab-G-transfected mammalian cells using RT-PCR, SDS-PAGE and Western blot analysis. The transfected cells also showed induction of apoptosis which is an important event in the enhancement of immune responses. Further, immune responses induced with replicon-based rabies DNA vaccine (pAlpha-Rab-G) was Compared with conventional rabies DNA vaccine and commercial cell Culture vaccine (Rabipur) in intramuscularly injected mice. The mice immunized with replicon-based rabies DNA vaccine induced humoral and cell mediated immune responses better than conventional rabies DNA vaccine however, comparable to Rabipur vaccine. On challenge with rabies virus CVS strain, replicon-based rabies DNA vaccine conferred complete protection similar to Rabipur. These results demonstrate that replicon-based rabies DNA vaccine is effective in inducing both humoral and cellular immune responses and can be considered as effective vaccine against rabies.

• 出版日期2008-12-2