MicroRNA and mRNA expression profile screening in multiple sclerosis patients to unravel novel pathogenic steps and identify potential biomarkers

作者：Martinelli Boneschi Filippo^*; Fenoglio Chiara; Brambilla Paola; Sorosina Melissa; Giacalone Giacomo; Esposito Federica; Serpente Maria; Cantoni Claudia; Ridolfi Elisa; Rodegher Mariaemma; Moiola Lucia; Colombo Bruno; De Riz Milena; Martinelli Vittorio; Scarpini Elio; Comi Giancarlo; Galimberti Daniela

来源：Neuroscience Letters, 2012, 508(1): 4-8.

DOI：10.1016/j.neulet.2011.11.006

• Multiple sclerosis
• MicroRNA
• Gene expression

Identification of novel targets and biomarkers, such as microRNAs, is extremely helpful to understand the pathogenetic mechanisms in a disease like multiple sclerosis (MS). We tested the expression profile of 1145 microRNAs in peripheral blood mononuclear cells (PBMCs) of 19 MS patients and 14 controls, and we further explored their function by performing a whole-genome mRNA profiling in same subjects and using bioinformatic prediction tool. A total of 104 miRNAs have been identified as deregulated in MS patients: 2/10 which ranked highest (let-7g and miR-150) have been validated in a replication sample, leading to the identification of putative target genes.

• 出版日期2012-2-2