Differential Intrasplenic Migration of Dendritic Cell Subsets Tailors Adaptive Immunity

作者：Calabro, Samuele; Liu, Dong; Gallman, Antonia; Nascimento, Manuela Sales L.; Yu, Zizi; Zhang, Ting-ting; Chen, Pei; Zhang, Biyan; Xu, Lan; Gowthaman, Uthaman; Krishnaswamy, Jayendra Kumar; Haberman, Ann M.; Williams, Adam^*; Eisenbarth, Stephanie C.^*

来源：Cell Reports, 2016, 16(9): 2472-2485.

DOI：10.1016/j.celrep.2016.07.076

• RED-BLOOD-CELLS
• CD4 T-CELLS
• IN-VIVO
• MARGINAL ZONE
• ANTIGEN PRESENTATION
• CROSS-PRESENTATION
• MOUSE SPLEEN
• WHITE PULP
• LYMPH-NODE
• CD8-ALPHA(+)

Evidence suggests that distinct splenic dendritic cell ( DC) subsets activate either CD4+ or CD8+ T cells in vivo. This bias has been partially ascribed to differential antigen presentation; however, all DC subsets can activate both T cell lineages in vitro. Therefore, we tested whether the organization of DC and T cell subsets in the spleen dictated this preference. We discovered that CD4+ and CD8+ T cells segregated within splenic T cell zones prior to immunization. After intravenous immunization, the two major conventional DC populations, distinguished by 33D1 and XCR1 staining, migrated into separate regions of the T cell zone: 33D1+ DCs migrated into the CD4+ T cell area, whereas XCR1+ DCs migrated into the CD8+ T cell area. Thus, the post-immunization location of each DC subset correlated with the T cell lineage it preferentially primes. Preventing this co-localization selectively impaired either CD4+ or CD8+ T cell immunity to blood-borne antigens.

• 出版日期2016-8-30
• 单位中山大学