Fibrinogen adsorption and platelet adhesion to silica surfaces with stochastic nanotopography

作者：Lord Megan S^*; Whitelock John M; Simmons Anne; Williams Rachel L; Milthorpe Bruce K

来源：Biointerphases: An Open Access Journal for the Biomaterials Interface Community , 2014, 9(4): 041002.

DOI：10.1116/1.4900993

In this study, the effect of surface nanoscale roughness on fibrinogen adsorption and platelet adhesion was investigated. Nanorough silica surfaces with a low level of surface roughness (10 nm R-rms) were found to support the same level of fibrinogen adsorption as the planar silica surfaces, while nanorough silica surfaces with higher levels of surface roughness (15 nm R-rms) were found to support significantly less fibrinogen adsorption. All surfaces analyzed were found to support the same level of platelet adhesion; however, platelets were rounded in morphology on the nanorough silica surfaces while platelets were spread with a well-developed actin cytoskeleton on the planar silica. Unique quartz crystal microbalance with dissipation monitoring (QCM-D) responses was observed for the interactions between platelets and each of the surfaces. The QCM-D data indicated that platelets were more weakly attached to the nanorough silica surfaces compared with the planar silica. These data support the role of surface nanotopography in directing platelet-surface interactions even when the adsorbed fibrinogen layer is able to support the same level of platelet adhesion.

• 出版日期2014-12