LIMITED HETEROGENEITY OF T-CELL RECEPTOR V-BETA GENE-EXPRESSION IN THE EARLY-STAGE OF INSULITIS IN NOD MICE

作者：DREXLER K; BURTLES S; HURTENBACH U

来源：Immunology Letters, 1993, 37(2-3): 187-196.

DOI：10.1016/0165-2478(93)90030-6

• NON-OBESE-DIABETIC MOUSE
• T-CELL RECEPTOR USAGE
• INSULITIS
• TYPE-I DIABETES

Non-obese-diabetic (NOD) mice spontaneously develop type I diabetes. The disease starts with T cells infiltrating the islets of Langerhans. We therefore examined the T-cell receptor (TCR) Vbeta region repertoire in islet infiltrates from individual female NOD mice from 4 to 10 week old by polymerase chain reaction (PCR) using Vbeta1-Vbeta17 specific oligonucleotides. The study revealed a limited heterogeneity of TCR Vbeta transcripts with a predominance of Vbeta1 at the onset of insulitis, i.e. at 4 weeks of age. The TCR VDJbeta sequences of the Vbeta1 PCR fragments were identical in most of the individual mice. Among several different mice, similarities in the beta-junctional regions were detected. In contrast, a large heterogeneity of TCR Vbeta usage was found in mice with advanced insulitis, i.e., from 6 weeks of age on. Thus, these data suggest a limited heterogencity of TCR Vbeta usage with a predominance of Vtbeta1 at the initiation phase of the disease.

• 出版日期1993-8