alpha-Lipoic acid reduces neurogenic hypertension by blunting oxidative stress-mediated increase in ADAM17

作者：de Queiroz Thyago M; Xia Huijing; Filipeanu Catalin M; Braga Valdir A; Lazartigues Eric^*

来源：American Journal of Physiology - Heart and Circulatory Physiology, 2015, 309(5): H926-H934.

DOI：10.1152/ajpheart.00259.2015

• metalloprotease 17
• deoxycorticosterone acetate-salt hypertension
• antioxidant
• type 2 angiotensin-converting enzyme

We previously reported that type 2 angiotensin-converting enzyme (ACE2) compensatory activity is impaired by the disintegrin and metalloprotease 17 (ADAM17), and lack of ACE2 is associated with oxidative stress in neurogenic hypertension. To investigate the relationship between ADAM17 and oxidative stress, Neuro2A cells were treated with ANG II (100 nM) 24 h after vehicle or alpha-lipoic acid (LA, 500 mu M). ADAM17 expression was increased by ANG II (120.5 +/- 9.1 vs. 100.2 +/- 0.8%, P < 0.05) and decreased after LA (69.0 +/- 0.3 vs. 120.5 +/- 9.1%, P < 0.05). In another set of experiments, LA reduced ADAM17 (92.9 +/- 5.3 vs. 100.0 +/- 11.2%, P < 0.05) following its overexpression. Moreover, ADAM17 activity was reduced by LA in ADAM17-overexpressing cells [109.5 +/- 19.8 vs. 158.0 +/- 20.0 fluorescence units (FU).min(-1).mu g protein(-1), P < 0.05], in which ADAM17 overexpression increased oxidative stress (114.1 +/- 2.5 vs. 101.0 +/- 1.0%, P < 0.05). Conversely, LA-treated cells attenuated ADAM17 overexpression-induced oxidative stress (76.0 +/- 9.1 vs. 114.1 +/- 2.5%, P < 0.05). In deoxycorticosterone acetate (DOCA)-salt hypertensive mice, a model in which ADAM17 expression and activity are increased, hypertension was blunted by pretreatment with LA (119.0 +/- 2.4 vs. 131.4 +/- 2.2 mmHg, P < 0.05). In addition, LA improved dysautonomia and baroreflex sensitivity. Furthermore, LA blunted the increase in NADPH oxidase subunit expression, as well as the increase in ADAM17 and decrease in ACE2 activity in the hypothalamus of DOCA-salt hypertensive mice. Taken together, these data suggest that LA might preserve ACE2 compensatory activity by breaking the feedforward cycle between ADAM17 and oxidative stress, resulting in a reduction of neurogenic hypertension.

• 出版日期2015-9-1