Non-toxic perfringolysin O and alpha-toxin derivatives as potential vaccine candidates against bovine necrohaemorrhagic enteritis

作者：Verherstraeten S; Goossens E; Valgaeren B; Pardon B; Timbermont L; Haesebrouck F; Ducatelle R; Deprez P; Van Immerseel F^*

来源：Veterinary Journal, 2016, 217: 89-94.

DOI：10.1016/j.tvjl.2016.09.008

• Calves
• Clostridium perfringens
• Necrohaemorrhagic enteritis
• theta-toxin
• Perfringolysin O
• alpha-toxin

Bovine necrohaemorrhagic enteritis is a fatal Clostridium perfringens type A-induced disease that is characterised by sudden death. Recently the involvement of perfringolysin O and alpha-toxin in the development of necrohaemorrhagic lesions in the gut of calves was suggested, and thus derivatives of these toxins are potentially suitable as vaccine antigens. In the current study, the perfringolysin O derivative PFOL491D, alone or in combination with alpha-toxin derivative GST-cpa(247-370), was evaluated as possible vaccine candidate, using in vitro assays.
PFOL491D showed no haemolytic effect on horse red blood cells and no cytotoxic effect on bovine endothelial cells. Furthermore, calves immunised with PFOL491D raised antibodies against perfringolysin that could inhibit the perfringolysin O-associated haemolytic activity on horse red blood cells. Antisera from calves immunised with PFOL491D had a significantly higher neutralising capacity against the cytotoxic effect of C. perfringens culture supernatant to bovine endothelial cells than serum from control calves (P < 0.05). Immunisation of calves with PFOL491D in combination with GST-cpa(247-370) elicited antibodies against perfringolysin O and alpha-toxin and consequently inhibited both the perfringolysin O-associated haemolytic activity and the alpha-toxin-associated lecithinase activity in vitro. Additionally, the neutralising ability of these antisera on the cytotoxic effect of C. perfringens culture supernatant to bovine endothelial cells was significantly higher than that from calves immunised with PFOL491D (P < 0.001). In conclusion, perfringolysin O derivative PFOL491D is an immunogenic antigen that can potentially be used to produce vaccine against bovine necrohaemorrhagic enteritis. Including alpha-toxin derivative GST-cpa(247-370) has an additional protective effect and therefore vaccination of calves with a combination of both antigens seems even more promising.

• 出版日期2016-11