Microscopy techniques in flavivirus research

作者：Chong Mun Keat; Chua Anthony Jin Shun; Tan Terence Tze Tong; Tan Suat Hoon; Ng Mah Lee^*

来源：Micron, 2014, 59: 33-43.

DOI：10.1016/j.micron.2013.12.006

• Flavivirus
• Fluorescence microscopy
• Transmission electron microscopy
• Scanning electron microscopy
• Correlative light electron microscopy
• Super resolution microscopy

The Flavivirus genus is composed of many medically important viruses that cause high morbidity and mortality, which include Dengue and West Nile viruses. Various molecular and biochemical techniques have been developed in the endeavour to study flaviviruses. However, microscopy techniques still have irreplaceable roles in the identification of novel virus pathogens and characterization of morphological changes in virus-infected cells. Fluorescence microscopy contributes greatly in understanding the fundamental viral protein localizations and virus-host protein interactions during infection. Electron microscopy remains the gold standard for visualizing ultra-structural features of virus particles and infected cells. New imaging techniques and combinatory applications are continuously being developed to push the limit of resolution and extract more quantitative data. Currently, correlative live cell imaging and high resolution three-dimensional imaging have already been achieved through the tandem use of optical and electron microscopy in analyzing biological specimens. Microscopy techniques are also used to measure protein binding affinities and determine the mobility pattern of proteins in cells. This chapter will consolidate on the applications of various well-established microscopy techniques in flavivirus research, and discuss how recently developed microscopy techniques can potentially help advance our understanding in these membrane viruses.

• 出版日期2014-4