Downregulation of matrix metalloproteinase-19 induced by respiratory syncytial viral infection affects the interaction between epithelial cells and fibroblasts

作者：Wu, Xiuxiu; Qi, Huijuan; Yang, Yan; Yin, Yunhong; Ma, Dedong; Li, Hao; Qu, Yiqing^*

来源：Molecular Medicine Reports, 2016, 13(1): 167-173.

DOI：10.3892/mmr.2015.4518

• airway hyper-responsiveness
• respiratory syncytial virus
• matrix metalloproteinase-19
• bronchial epithelial cells
• epithelial-mesenchymal transition
• lung fibroblast

The present study was designed to examine the expression and function of matrix metalloproteinase-19 (MMP-19), which is downregulated following respiratory syncytial virus (RSV) infection. The diverse expression levels of MMP were examined using a designed cDNA expression array. The expression and secretion of MMP-19 was examined using reverse transcription-quantitative polymerase chain reaction (RT-qPCR) analysis and ELISA, respectively. The proliferation of epithelial cells and lung fibroblasts were examined using flow cytometry. The epithelial-mesenchymal transition (EMT) was also examined by performing western blot and RT-qPCR analyses. The results of the cDNA assay showed that infection with RSV resulted in the abnormal expression of certain metalloproteinases. Among these, the expression of MMP-19 decreased 3 and 7 days following infection. By using flow cytometric, western blot and RT-qPCR analyses, the present study demonstrated that the downregulation of MMP-19 inhibited the proliferation of epithelial cells, promoted the EMT and induced the proliferation of lung fibroblasts. Taken together, the findings of the present study suggested that the downregulation of MMP-19 following RSV infection may be associated with the development of airway hyper-responsiveness.

• 出版日期2016-1
• 单位山东大学