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摘要
The genetic diversity and genetic relationships of 46 Osmanthus fragrans cultivars, collected from Hubei, Zhejiang, and Guangxi provinces in China, were analyzed by the technique of amplified fragment length polymorphism (AFLP). Ten primer combinations were used and generated 436 scorable bands including 269 polymorphic. It indicated that each primer combination generated 26.9 polymorphic bands. Genetic similarities were obtained using simple matching (SM) coefficients, and a dendrogram of the 46 cultivars was established by UPGMA clustering method. The high level genetic variations in 46 O. fragrans cultivars were proved by the SM coefficient value from 0.69 to 0.87. The cluster analysis suggested that the 46 O. fragrans cultivars could be divided into five groups, and this result was not absolutely consistent with the morphology-based traditional classification. The cluster analysis showed that there were close genetic relationships among cultivars of the same flower color, and the geographic origin of O. fragrans was correlated with the analysis cluster results at a certain level. Cluster analysis also indicated that Guangxi cultivars were distinct from those of Hubei and Zhejiang. [Life Science Journal. 2009; 6(2): 11 - 16] (ISSN: 1097 - 8135).
- 出版日期2009
- 单位河南大学
