A NAD-dependent glutamate dehydrogenase coordinates metabolism with cell division in Caulobacter crescentus

作者：Beaufay Francois; Coppine Jerome; Mayard Aurelie; Laloux Geraldine; De Bolle Xavier; Hallez Regis^*

来源：The EMBO Journal, 2015, 34(13): 1786-1800.

DOI：10.15252/embj.201490730

• cell division
• cytokinesis
• FtsZ
• GdhZ
• glutamate dehydrogenase

Coupling cell cycle with nutrient availability is a crucial process for all living cells. But how bacteria control cell division according to metabolic supplies remains poorly understood. Here, we describe a molecular mechanism that coordinates central metabolism with cell division in the alpha-proteobacterium Caulobacter crescentus. This mechanism involves the NAD-dependent glutamate dehydrogenase GdhZ and the oxidoreductase-like KidO. While enzymatically active GdhZ directly interferes with FtsZ polymerization by stimulating its GTPase activity, KidO bound to NADH destabilizes lateral interactions between FtsZ protofilaments. Both GdhZ and KidO share the same regulatory network to concomitantly stimulate the rapid disassembly of the Z-ring, necessary for the subsequent release of progeny cells. Thus, this mechanism illustrates how proteins initially dedicated to metabolism coordinate cell cycle progression with nutrient availability.

• 出版日期2015-7-2