Anti-botulism single-shot vaccine using chitosan for protein encapsulation by simple coacervation

作者：Sari Roger S; de Almeida Anna Christina; Cangussu Alex S R; Jorge Edson V; Mozzer Otto D; Santos Hercules Otacilio; Quintilio Wagner; Brandi Igor Viana; Andrade Viviane Aguiar; Miguel Angelo Samir M; Sobrinho Santos Eliane M

来源：Anaerobe, 2016, 42: 182-187.

DOI：10.1016/j.anaerobe.2016.10.013

• Single-shot vaccine
• Chitosan
• Simple coacervation
• Controlled antigen release
• Neurotoxins

The aim of the present study was to compare the potency and safety of vaccines against Clostridium botulinum (C. botulinum) type C and D formulated with chitosan as controlled release matrix and vaccines formulated in conventional manner using aluminum hydroxide. Parameters were established for the development of chitosan microspheres, using simple coacervation to standardize the use of this polymer in protein encapsulation for vaccine formulation. To formulate a single shot vaccine inactivated antigens of C botulinum type C and D were used with original toxin titles equal to 5.2 and 6.2 log LD50/ml, respectively. For each antigen a chitosan based solution of 50 mL was prepared. Control vaccines were formulated by mixing toxoid type C and D with aluminum hydroxide [25% Al(OH)(3), pH 6.3]. The toxoid sterility, innocuity and potency of vaccines were evaluated as stipulated by MAPA-BRASIL according to ministerial directive no. 23. Encapsulation efficiency of BSA in chitosan was 32.5-40.37%, while that the encapsulation efficiency to toxoid type C was 41,03% (1.94 mg/mL) and of the toxoid type D was 32.30% (1.82 mg/mL).
The single shot vaccine formulated using chitosan for protein encapsulation through simple coacervation showed potency and safety similar to conventional vaccine currently used in Brazilian livestock (10 and 2 IU/mL against C botulinum type C and D, respectively). The present work suggests that our single shot vaccine would be a good option as a cattle vaccine against these C botulinum type C and D.

• 出版日期2016-12