科研之友
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摘要
Signal transducer and activator of transcription 4 (STAT4) belongs to the STATs family which may play important roles in the activation of milk protein genes and the development of mammary glands. Four novel single nucleotide polymorphisms (SNPs) (g. 2624A>C ss175327225, g. 60330A>G ss175327226, g. 63823G>C ss175327227, g. 66912C>T ss175327228) of the STAT4 gene were investigated in 966 cattle of three breeds (Chinese holstein, Luxi yellow and Bohai black cattle) in China by polymerase chain reaction-single-strand conformation polymorphism (PCR-SSCP), PCR-restriction fragment length polymorphism (RFLP) and DNA sequencing methods. The SNP (g. 60330A>G ss175327226) could be genotyped into three genotypes (AA, AG and GG) with PCR-RFLP using Msp I and another SNP (g. 66912C>T ss175327228) only into two genotypes (CC and TC) by PCR-SSCP. The allelic frequencies of the SNP (g. 60330A>G) were different among the three different cattle breeds (P < 0.05). The associations between polymorphisms of the STAT4 gene and dairy performance traits were analyzed in 793 Chinese holstein cows. The SNP (g. 60330A>G) markedly affected the 305 d matured equivalency (P < 0.05) and fat content (P < 0.05). However, the SNP (g. 66912C>T) had no significant relationship with the milk production traits (P > 0.05). Together, the SNP (g. 60330A>G) of the STAT4 gene may serve as a molecular marker for the differentiation of various cattle populations and selection of the milk yield and fat content in bovine breeding program.
