Adsorption of nisin on raw montmorillonite

作者：Ibarguren Carolina; Naranjo Pablo M; Stoetzel Christian; Carina Audisio M; Sham Edgardo L; Farfan Torres E Monica; Mueller Frank A

来源：Applied Clay Science, 2014, 90: 88-95.

DOI：10.1016/j.clay.2013.12.031

• Nisin
• Montmorillonite
• Peptide/support interaction
• Food biopreservative

The aim of the present work was to develop food-grade materials capable of releasing antimicrobial agents. Montmorillonite (Mt), a raw, abundant inexpensive clay mineral approved as a food additive, was used as support material. The immobilized antimicrobial agent was pure nisin (Danisco, UK). A series of antibacterial compounds were prepared, and the antimicrobial activity of the resultant montmorillonite-nisin (MtNis) immobilized systems was determined against Enterococcus faecium Cl. The characterization of the antimicrobial powders showed evidences of nisin adsorption on by means of a %26quot;frustrated intercalation%26quot; model with a nisin loading (NL) saturation value close to 0.24 CEC (cation exchange capacity) of Mt. The antibacterial powders with NL above this value completely inhibited E. faecium Cl during 24 h of contact These results are encouraging for the development of nisin-based bioprotectors immobilized on raw Mt.

• 出版日期2014-3