Alkylation-induced genotoxicity as a predictor of DNA repair deficiency following experimental oral carcinogenesis

作者：Carvalho Juliana G; Noguti Juliana; da Silva Victor Hugo P; Dedivitis Rogerio A; Franco Marcello; Ribeiro Daniel A^*

来源：Journal of Molecular Histology, 2012, 43(2): 145-150.

DOI：10.1007/s10735-011-9388-5

• Rat tongue mucosa
• Oral squamous cell carcinoma
• 4-Nitroquinoline 1-oxide
• DNA repair deficiency
• Methylmetanesulfonate

The aim of this study was to evaluate alkylation induced genotoxicity as a result of DNA repair deficiency during 4-nitroquinoline 1-oxide (4NQO)-induced rat tongue carcinogenesis by means of single cell gel (comet) assay. Male Wistar rats were distributed into three groups of 10 animals each and treated with 50 ppm 4NQO solution through their drinking water for 4, 12, and 20 weeks. Ten animals were used as negative control. Blood samples and oral mucosa cells collected from all animals were divided into two aliquots of 20 mu L each to study basal DNA damage and DNA damage due to genotoxin sensitivity. The first aliquot was processed immediately for comet assay to assess basal DNA damage. The second aliquot was treated with a known genotoxin, methylmetanesulfonate. Significantly greater DNA damage was noticed to oral mucosa cells from 4, and 12 weeks post-treatment. Peripheral blood cells did show statistically significant differences (P < 0.05) after 20 weeks-group (squamous cell carcinoma). In conclusion, alkylation induced genotoxicity as a result of DNA repair deficiency is present in oral mucosa cells following oral experimental carcinogenesis.

• 出版日期2012-4