Scanning electrochemical microscopy as a readout tool for protein electrophoresis

作者：Zhang Meiqin; Wittstock Gunther; Shao Yuanhua; Girault Hubert H^*

来源：Analytical Chemistry, 2007, 79(13): 4833-4839.

DOI：10.1021/ac062356k

Scanning electrochemical microscopy (SECM) was used to image silver-stained proteins on a poly(vinylidene difluoride) membrane. The method is based on measuring the current at a scanning microelectrode in the feedback mode. The electrochemical feedback is caused by the redox-mediated etching of the isolated 5-10-nm-diameter silver nanoparticles formed during the staining process. Several parameters, such as the redox mediator and the staining protocol, were optimized to ensure a high resolution and a low detection limit, i.e., 0.5 ng of bovine serum albumin (4 x 10(-14) mol) distributed on an area of 1 mm(2) (4 x 10(-16) mol center dot cm(-2)). Images of beta-lactoglobulin A and myoglobin bands after gel electrophoretic separation and electroblotting were obtained in order to demonstrate that SECM can be employed as a sensitive and quantitative readout method for detection of proteins after gel electrophoresis. An additional advantage is that the silver staining can be removed, allowing further downstream mass spectrometry analysis.

• 出版日期2007-7-1
• 单位北京大学