SOLID-STATE FERMENTATION OF BREWER'S SPENT GRAIN FOR XYLANOLYTIC ENZYMES PRODUCTION BY Penicillium janczewskii AND ANALYSES OF THE FERMENTED SUBSTRATE

作者：Fanchini Terrasan Cesar Rafael; Carmona Eleonora Cano

来源：Bioscience Journal, 2015, 31(6): 1826-1836.

DOI：10.14393/bj-v31n6a2015-30044

• Enzyme production
• Xylanolytic enzymes
• Penicillium janczewskii
• Solid-state fermentation
• Brewer's spent grain

In recent decades, increasing interest has been devoted to xylanolytic enzymes due to their potential use in many industrial processes. This study describes the production of xylanase, beta-xylosidase and alpha-L-arabinofuranosidase, belonging to the xylanolytic complex, by Penicillium janczewskii using brewer's spent grain as substrate for solid-state fermentation. The optimized conditions for high levels of xylanase, beta-xylosidase and alpha-L-arabinofuranosidase production were: 50% initial moisture, which was provided by Vogel's salt solution, seven days of cultivation at 20-30 degrees C. Fermentation enriched the bioproduct with some amino acids and did not add mycotoxins to it. The use of brewer's spent grain as substrate for fungal cultivation and enzyme production can both add value to this waste and reduce the production cost of xylanolytic enzymes.

• 出版日期2015-12