A Validated LC-MS/MS Method for Simultaneous Determination of Six Aconitum Alkaloids and Seven Ginsenosides in Rat Plasma and Application to Pharmacokinetics of Shen-Fu Prescription

作者：Ouyang, Huizi; Liu, Fang; Tang, Zhidan; Chen, Xiaopeng; Bo, Fang; Liu, Huaming; Chang, Yanxu; He, Jun^*

来源：Evidence-Based Complementary and Alternative Medicine, 2018, 2018: 5107083.

DOI：10.1155/2018/5107083

• LIQUID-CHROMATOGRAPHY
• MASS-SPECTROMETRY
• RADIX-ACONITI
• GINSENG
• LATERALIS
• COMBINATION
• INJECTION
• TOXICITY

A sensitive and reliable LC-MS/MS method has been developed and validated for simultaneous determination of six Aconitum alkaloids (aconitine, hypaconitine, mesaconitine, benzoylaconitine, benzoylhypacoitine, and benzoylmesaconine) and seven ginsenosides (Rb-1, Rb-2, Rc, Rd, Re, Rf, and Rg(1)) in rat plasma after oral administration of Shen-Fu prescription. Psoralen was selected as internal standard (IS). Protein precipitation with methanol was used in sample preparation. The chromatographic separation was achieved on a CORTECS (TM) C18 column with 0.1% formic acid aqueous solution and acetonitrile as mobile phase. The flow rate was 0.3 mL/min. The detection was performed on a tandem mass system with an electrospray ionization (ESI) source in the positive ionization and multiple-reaction monitoring (MRM) mode. The calibration curves of six Aconitum alkaloids and seven ginsenosides were linear over the range of 0.1-50 and 1-500 ng/mL, respectively. The extraction recoveries of the analytes in plasma samples ranged from 64.2 to 94.1%. Meanwhile, the intra- and interday precision of the analytes were less than 14.3%, and the accuracy was in the range of -14.2% to 9.8%. The developed method was successfully applied to the pharmacokinetics of six Aconitum alkaloids and seven ginsenosides in rat plasma after oral administration of Shen-Fu prescription.

• 出版日期2018
• 单位天津中医药大学