An immediate-early protein of white spot syndrome virus modulates the phosphorylation of focal adhesion kinase of shrimp

作者：Lu Huasong; Ruan Lingwei; Xu Xun^*

来源：Virology, 2011, 419(2): 84-89.

DOI：10.1016/j.virol.2011.07.021

• Shrimp
• WSSV
• WSV083
• Focal adhesion kinase
• Phosphorylation

WSSV interacts with integrin during infection of shrimps and modulate the focal adhesion kinase which is known as a regulator of several downstream signaling pathways. Viral protein kinases are thought to be important for virus infection by regulating the host signaling pathways. WSV083 is an immediate-early gene of white spot syndrome virus that contains a Ser/Thr protein kinase domain. So, does WSSV modulate FAR phosphorylation via the WSV083 molecule? In this study, co-transfection of WSV083 and MjFAK genes proceeded in insect cells revealed that the MjFAK phosphorylation and cell adhesion activity could be inhibited by the expression of WSV083. Kinase domain mutants of WSV083 lost its ability of inhibiting FAK phosphorylation. Moreover, silencing of FAK gene through RNAi accelerated the shrimp death rate upon WSSV challenge. These results demonstrate for the first time that modulation of FAR phosphorylation by WSV083 plays a critical role in the pathogenesis of WSSV infection.

• 出版日期2011-10-25
• 单位国家海洋局第三海洋研究所