Anticancer Activity of Kalanchoe tubiflora Extract Against Human Lung Cancer Cells In Vitro and In Vivo

作者：Hsieh Yi Jen; Huang Hsuan Shun; Leu Yann Lii; Peng Kou Cheng; Chang Chih Jui; Chang Meng Ya^*

来源：Environmental Toxicology, 2016, 31(11): 1663-1673.

DOI：10.1002/tox.22170

• Kalanchoe tubiflora
• H2O-soluble fraction
• lung cancer
• tumor growth inhibition
• cell cycle arrest
• senescence
• A549 xenograft model

Uncontrolled cell proliferation is a common feature of human cancer. Some of herbal extract or plant-derived medicine had been shown as an important source of effective anticancer agents. We previously reported that an n-BuOH-soluble fraction of Kalanchoe tubiflora has antiproliferative activity by inducing mitotic catastrophe. In this study, we showed that the H2O-soluble fraction of Kalanchoe tubiflora (KT-W) caused cell cycle arrest, and senescence-inducing activities in A549 cells. We used 2 dimensional PAGE to analyze the protein expression levels after KT-W treatment, and identified that the energy metabolism-related proteins and senescence-related proteins were disturbed. In vivo experiments showed that the tumor growths in A549-xenografted nude mice were effectively inhibited by KT-W. Our findings implied that KT-W is a putative antitumor agent by inducing cell cycle arrest and senescence.

• 出版日期2016-11
• 单位东华大学; 河北医科大学; 长春大学