Nanoscale Biomolecular Structures on Self-Assembled Monolayers Generated from Modular Pegylated Disulfides

作者：Wong, Lu Shin; Janusz, Stefan J.; Sun, Shuqing; Leggett, Graham J.; Micklefield, Jason^*

来源：Chemistry - A European Journal, 2010, 16(40): 12234-12243.

DOI：10.1002/chem.200902439

• bionanotechnology
• monolayers
• photolithography
• protein immobilization
• self-assembly
• solid-phase synthesis

A solid-phase synthetic strategy was developed that uses modular building blocks to prepare symmetric oligo(ethylene glycol)-terminated disulfides with a variety of lengths and terminal functionalities. The modular disulfides, composed of alkyl amino groups linked by an amide group to oligoethylene chains were used to generate self-assembled monolayers (SAMs), which were characterised to determine their applicability for biomolecular applications. X-ray photo-electron spectroscopy (XPS) of the SAMs obtained from these molecules demonstrated improved stability towards displacement by 16-hexadecanethiol, while surface plasmon resonance (SPR) analyses of SAMs prepared with the hydroxy-terminated oligoethylene disulfide showed equal resistance to non-specific protein adsorption in comparison to 11-mercaptoundecyl tri(ethylene glycol). SAMs made from these adsorbates were amenable to nanoscale patterning by scanning near-field photolithography (SNP), facilitating the fabrication of nanopatterned, protein-functionalised surfaces. Such SAMs may be further developed for bionanotechnology applications such as the fabrication of nanoscale biological arrays and sensor devices.

• 出版日期2010
• 单位国家纳米科学中心