Ubp15p, a Ubiquitin Hydrolase Associated with the Peroxisomal Export Machinery

作者：Debelyy Mykhaylo O; Platta Harald W; Saffian Delia; Hensel Astrid; Thoms Sven; Meyer Helmut E; Warscheid Bettina; Girzalsky Wolfgang; Erdmann Ralf^*

来源：Journal of Biological Chemistry, 2011, 286(32): 28223-28234.

DOI：10.1074/jbc.M111.238600

Peroxisomal matrix protein import is facilitated by cycling receptors shuttling between the cytosol and the peroxisomal membrane. One crucial step in this cycle is the ATP-dependent release of the receptors from the peroxisomal membrane. This step is facilitated by the peroxisomal AAA (ATPases associated with various cellular activities) proteins Pex1p and Pex6p with ubiquitination of the receptor being the main signal for its export. Here we report that the AAA complex contains dislocase as well as deubiquitinating activity. Ubp15p, a ubiquitin hydrolase, was identified as a novel constituent of the complex. Ubp15p partially localizes to peroxisomes and is capable of cleaving off ubiquitin moieties from the type I peroxisomal targeting sequence (PTS1) receptor Pex5p. Furthermore, Ubp15p-deficient cells are characterized by a stress-related PTS1 import defect. The results merge into a picture in which removal of ubiquitin from the PTS1 receptor Pex5p is a specific event and might represent a vital step in receptor recycling.

• 出版日期2011-8-12