Adenosine 3 ',5 '-Cyclic Monophosphate Involvement in Hepatic Triacylglyceride Lipase Release from Prazosin-Stimulated Primary Cultured Rat Hepatocytes

作者：Nakamura Tetsuya^*; Morita Tetsuo

来源：Biological & Pharmaceutical Bulletin, 2014, 37(6): 922-925.

DOI：10.1248/bpb.b13-00798

• prazosin
• hepatic lipase
• cAMP
• protein kinase A
• hepatocyte

We recently found that hepatic triglyceride lipase (HTGL) was released from primary cultured rat hepatocytes after treatment with prazosin, an antagonist of alpha-1 adrenoceptors. However, the details of prazosin-induced HTGL release remain uncertain. Here we investigated whether changes in cAMP levels in hepatocytes were related to HTGL release from prazosin-stimulated hepatocytes. When hepatocytes were treated with prazosin, cAMP levels during stimulated release of HTGL increased in a time- and dose-dependent manner. Stimulated release of HTGL was suppressed by the adenylate cyclase inhibitors MDL-12,330A and 2',5'-dideoxyadenosine. Further, cAMP-dependent protein kinase A (PKA) activity in prazosin-stimulated hepatocytes also increased in a time- and dose-dependent manner. Moreover, prazosin-stimulated HTGL release was suppressed by the PKA inhibitors H-89 and KT5720. These results suggest that prazosin-stimulated HTGL release from hepatocytes was due to cAMP production and partly due to subsequent PKA activation in hepatocytes.

• 出版日期2014-6