Molecular characterization and biological activities of watersoluble sulfated polysaccharides from Enteromorpha prolifera

作者：Cho MyoungLae; Yang Chen; Kim Sang Moo; You SangGuan^*

来源：Food Science and Biotechnology, 2010, 19(2): 525-533.

DOI：10.1007/s10068-010-0073-3

• Enteromorpha prolifera
• sulfated polysaccharide
• molecular weight
• anticancer activity
• nitrite release

Sulfated polysaccharides isolated from Enteromorpha prolifera and fractionated using ion exchange and gel permeation chromatography were investigated to determine their molecular characteristics and biological activities. The crude and fractionated polysaccharides (F(1), F(2), and F(3)) consisted mostly of carbohydrates (50.8-62.5%), sulfates (14.5-18.8%), and uronic acid (13.8-16.6%) with different levels of monosaccharides, including rhamnose (57.1-87.6%), glucose (3.6-39.1%), and xylose (2.4-8.8%). The protein content ranged from 1.0 to 13.9%, and after the fractionation, most proteins were included in the F(2) fraction (11.3%). The crude, F(1), F(2), and F(3) exhibited the molecular weights (Mw) of 37-1,281x10(3) g/mol. The polysaccharides had no significant direct cytotoxicity to cancer cells AGS or DLD- 1. On the other hand, the F(1) and F(2) stimulated Raw 264.7 cells to produce a considerable amount of nitric oxide (NO). The investigation of F(2) subfractions failed to reveal a specific subfraction more significantly affecting the NO production, thus requiring further systematic elucidation of their structural characteristics.

• 出版日期2010-4