Detecting of Benzo[a]pyrene Using a Label-free Amperometric Immunosensor

作者：Zhang, Yanhua; Su, Qing; Xu, Junhui; Zhang, Yan; Chen, Songtao

来源：International Journal of Electrochemical Science, 2014, 9(7): 3736-3745.

• Prussian blue
• Gold nanoparticles
• L-cysteine
• Amperometric immunosensor
• Benzo[a]pyrene

A label-free amperometric immunosensor was manufactured for the fast and sensitive detection of Benzo[a]pyrene. Prussian blue was firstly electrodeposited onto a glass carbon electrode as an electron mediator. Afterwards nano-gold layer was formed on the prussian blue film by electrochemical reduction of HAuCl4 solution. Then, L-cysteine and nano-gold particles were self-assembled layer-by-layer. Finally, anti-benzo[a]pyrene was adsorbed onto the bilayer nano-gold films to construct a sensitive immunosensor. Under the optimal experimental condition, the current response of the immunosensor was inversely proportional to the concentration of benzo[a]pyrene in the range of 0.2 similar to 100 ng.mL(-1) with a detection limit of 0.08 ng.mL(-1). The reported immunosensor exhibited a long-term stability and was easy to be regenerated. This method was applied to the benzo[a]pyrene detection in waste water samples with satisfactory results.

• 出版日期2014-7
• 单位河南城建学院