A novel avian retrovirus associated with lymphocytoma isolated from a local Chinese flock induced significantly reduced growth and immune suppression in SPF chickens

作者：Wu, Xiaoping^*; Zhao, Jinrong; Zeng, Yukun; Wu, Yijian; Wang, Quanxi; Wu, Baocheng; Huang, Yifan

来源：Veterinary Microbiology, 2017, 205: 34-38.

DOI：10.1016/j.vetmic.2017.04.011

• Avian leukosis virus
• Lymphocytoma
• Growth and immune suppression
• Chinese local flock

Avian Leukosis Viruses (ALVs) are associated with neoplasias, immune suppression and reduced performance in chicken flocks. In the present study, a naturally occurring recombinant strain of ALV (FJ15HT0) was isolated from an infected flock of Chinese "Hetian" chickens, and was subsequently identified as an exogenous ALV by immuno-fluorescence assay (IFA), PCR and following entire proviral DNA nucleotide sequencing. This isolate is revealed as a novel recombinant virus, lacking viral oncogenes, with the gp85 (93.4%) of subgroup B, the U3 (92.1%) and R (95.2%) region of subgroup J, the U5 (93.8%) region and 5'UTR (95.7%) of subgroup C, as well as the gp37 (90.6%) and 3' (92.2%) of ALV-E. The simulative congenital infection with this isolate in SPF chickens resulted in significant weight loss (P < 0.05) and a significant reduction in the humoral immune response to the live NDV vaccine (P < 0.05), but not to the inactive AIV-H5 vaccine (P > 0.05). Foci of lymphocytomas were observed in tissues of congenitally infected chickens at 11 weeks post-hatch, demonstrating the acute oncogenicity of the isolate.

• 出版日期2017-6
• 单位福建农林大学