CircBAGE2(hsa＿circ＿0061259) regulates CCND1 and PDCD10 expression by functioning as an miR-103a-3p 'sponge' to alter the proliferation and apoptosis of prostate cancer cells

作者：Chunlei Zhang; Dan Liu; Qinqin Tian; Qi Yang; Xiaoyuan Zi^*; Yinghao Sun^*

来源：肿瘤学与转化医学(英文), 2021, 7(05): 221-228.

Objective The aim of the article is to explore the function of circBAGE2 (hsacirc0061259) in prostate cancer (PCa) cells.Methods Sequencing results of circBAGE2 were verified by quantitative RT PCR (qRT-PCR) and Sanger sequencing.Agarose gel electrophoresis was used to detect the resistance of GAPDH,BAGE2,and circBAGE2 to RNase R and their expression as cDNA and gDNAin 22RV1 cells.The biological functions of circBAGE2 were investigated by CCK8 assay and flow cytometry in 22RV1 cells transfected with siRNAs.Multiple databases were used to predict the target binding sites between circRNAs,miRNAs,and mRNAs.Western blotting was used to detect the expression of CCND1 and PDCD10.Results CircBAGE2 was significantly upregulated in PCa samples and PCa cells compared to that in matched normal tissues and normal cells,and CircBAGE2 knockdown inhibits cell proliferation and promotes apoptosis.Downregulation of circBAGE2 compromised the expression of CCND1 and PDCD10.The 3’ UTRs of CCND1 and PDCD10 were matched by miR-103a-3p,which shared binding sites with circBAGE2.Conclusion CircBAGE2 contributes to PCa progression by upregulating CCND1 and PDCD10 expression through its role as a ‘sponge’ of miR-103a-3p.CircBAGE2 may be a potential therapeutic target for PCa.

• 出版日期2021