Activation of intrarenal complement system in mouse model for chronic cyclosporine nephrotoxicity

作者：Kim Young Ok; Lim Sun Woo; Li Can; Kang Hee Jung; Ahn Kyung Ohk; Yang Hyun Joo; Ghee Jung Yeon; Kim Su hyun; Kim Jin Young; Choi Bum Soon; Kim Jin; Yang Chul Woo^*

来源：Yonsei Medical Journal, 2007, 48(3): 517-525.

DOI：10.3349/ymj.2007.48.3.517

• cyclosporine
• complement
• nephrotoxicity
• innate immunity

Purpose: Local activation of the complement system plays a role in target organ damage. The aim of our study was to investigate the influence of cyclosporine (CsA)- induced renal injury on the complement system in the kidney. Materials and Methods: Mice fed a low salt (0.01%) diet were treated with vehicle (VH, olive oil, I mL/kg/day) or CsA (30 mg/ kg/day) for one or four weeks. Induction of chronic CsA nephrotoxicity was evaluated with renal function and histomorphology. Activation of the complement system was assessed through analysis of the expression of C3, C4d, and membrane attack complex (MAC), and the regulatory proteins, CD46 and CD55. CsA treatment induced renal dysfunction and typical morphology (tubulointerstitial inflammation and fibrosis) at four weeks. Results: CsA-induced renal injury,vas associated with increased the expression of C3, C4d, and MAC (C9 and upregulation of complement regulatory proteins (CD 46 and CD55). Irnmunohistochemistry revealed that the activated complement components were mainly confined to the inj tired tubulointerstitium. Conclusion: CsA-induced renal ir jury is associated with activation of the intrarenal complement system.

• 出版日期2007-6-30
• 单位延边大学