Leukotriene B-4 receptors BLT1 and BLT2 are involved in interleukin-8 production in human neutrophils induced by Trichomonas vaginalis-derived secretory products

作者：Nam Young Hee; Min Arim; Kim Seong Hoon; Lee Young Ah; Kim Kyeong Ah; Song Kyoung Ju; Shin Myeong Heon^*

来源：Inflammation Research, 2012, 61(2): 97-102.

DOI：10.1007/s00011-011-0425-3

• Trichomonas vaginalis
• Human neutrophils
• IL-8
• Leukotriene B-4 receptors BLT1
• BLT2

Trichomonas vaginalis is a flagellated protozoan parasite that causes human trichomoniasis. Although T. vaginalis itself can secrete lipid mediator leukotriene (LT) B-4 leading to neutrophil activation, information regarding the signaling mechanism involved in neutrophil activation induced by T. vaginalis-secreted LTB4 is limited. We investigated whether LTB4 contained in the T. vaginalis-derived secretory products (TvSP) is closely involved in interleukin (IL)-8 production in human neutrophils via LTB4 receptors BLT1 or BLT2.
T. vaginalis produced more than 714 pg/ml of LTB4 per 1 x 10(7) trichomonads. The ability of trichomonads to secrete LTB4 was inhibited by treatment of trichomonads with the 5-lipo-oxygenease inhibitor AA861, but not the cyclo-oxygenease I inhibitor FR122047. When neutrophils were incubated with TvSP obtained from 1 x 10(7) trichomonads, IL-8 protein secretion was significantly increased compared to results for cells incubated with medium alone. The stimulatory effect of TvSP on IL-8 production was strongly inhibited by pretreatment of TvSP with lipase, although pretreatment with heat or proteinase K showed little inhibitory effect. Moreover, TvSP-induced IL-8 production was efficiently inhibited when trichomonads were pretreated with AA861 or when neutrophils were pretreated with antagonists for BLT1 or BLT2.
Our results suggest that LTB4 receptors BLT1 and BLT2 are involved in IL-8 production in neutrophils induced by T. vaginalis.

• 出版日期2012-2