Nucleocytoplasmic Coagulation: An Injury-Induced Aggregation Event that Disulfide Crosslinks Proteins and Facilitates Their Removal by Plasmin

作者：Samson Andre L; Knaupp Anja S; Sashindranath Maithili; Borg Rachael J^*; Au Amanda E L; Cops Elisa J; Saunders Helen M; Cody Stephen H; McLean Catriona A; Nowell Cameron J; Hughes Victoria A; Bottomley Stephen P; Medcalf Robert L

来源：Cell Reports, 2012, 2(4): 889-901.

DOI：10.1016/j.celrep.2012.08.026

Cellular injury causes a myriad of processes that affect proteostasis. We describe nucleocytoplasmic coagulation (NCC), an intracellular disulfide-dependent protein crosslinking event occurring upon late-stage cell death that orchestrates the proteolytic removal of misfolded proteins. In vitro and in vivo models of neuronal injury show that NCC involves conversion of soluble intracellular proteins, including tubulin, into insoluble oligomers. These oligomers, also seen in human brain tissue following neurotrauma, act as a cofactor and substrate for the plasminogen-activating system. In plasminogen(-/-) mice, levels of misfolded beta-tubulin were elevated and its clearance delayed following neurotrauma, demonstrating a requirement for plasminogen in the removal of NCC constituents. While additional in vivo studies will further dissect this phenomenon, our study clearly shows that NCC, a process analogous to the formation of thrombi, generates an aggregated protein scaffold that limits release of cellular components and recruits clearance mechanisms to the site of injury.

• 出版日期2012-10