科研之友
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摘要
Protein phosphatase 2C (PP2C), one of the protein serine/threonine phosphatases, plays a negative role in plant development, organ formation, hormone regulation and stress resistance. However, fewer studies have shown that the PP2C gene is positively regulated by abiotic stresses in plants. In this study, one PP2C gene, MsPP2C, was isolated from Medicago sativa L. cv. 'Gongnong No. 2' (alfalfa Zhongmu). MsPP2C contains an 1176-bp open reading frame that encodes a 391-amino acid protein. Fluorescence quantitative PCR showed that salt stress, abscisic acid (ABA), and drought stress treatments induced the expression of MsPP2C. To further investigate the salt-stress resistance in the MsPP2C-overexpressing plants, 35S:MsPP2C transgenic Arabidopsis plants were obtained by transforming a constructed vector through Agrobacterium-mediated transformation. Compared with the wild type (WT) line, in the MsPP2C-overexpressing plants, Fv/Fm increased by 50-52%, the chlorophyll content elevated by 31-63%, the MDA content decreased by 17-21%, and the proline content increased by 17-23% under salt stress. Additionally, the sodium ion (Na+) content in both the leaves and roots increased. However, the aboveground tissues in the transgenic plants had a lower Na+ content, while the roots had a higher Na+ content. In conclusion, our results suggest that the overexpression of the MsPP2C gene isolated from alfalfa Zhongmu conferred salt-tolerance in Arabidopsis. The salt tolerance may due to osmolytes accumulation in the expanded cells instead of protection of the cell membrane from the damage by the accumulation of superoxide anion radicals. This study provides a basis for improving biotechnological approaches for alfalfa to adapt salt stress driven by probably future climate change.
- 出版日期2018
- 单位大连民族大学; 大连市农业科学研究院
