Antitumor progression potential of morusin suppressing STAT3 and NF kappa B in human hepatoma SK-Hep1 cells

作者：Lin Wea Lung; Lai Deng Yu; Lee Yean Jang^*; Chen Nai Fang; Tseng Tsui Hwa

来源：Toxicology Letters, 2015, 232(2): 490-498.

DOI：10.1016/j.toxlet.2014.11.031

• Hepatoma
• SK-Hep 1?cells
• NF kappa B
• Morusin
• STAT3
• Tumor progression

Morusin is a prenylated flavonoid that has been isolated from the root bark of the mulberry tree (Morus species, Moraceae), a Chinese traditional medicine. It has been synthesized by our laboratory from commercially available phloroglucinol, and has demonstrated to possess antitumor effects of cell lines including A549, MCF-7, and MDA-MB-231. In this study, at non-cytotoxic concentrations, morusin altered invasive morphology and suppressed cell-matrix adhesion, cell motility and cell invasion in SK-Hep1 cells. Morusin also increased the expression of E-cadherin, an epithelial cell junction protein, decreased the expression of vimentin, a mesecnchymal marker, and alpha 2-, alpha 6-, beta 1- integrin, which regulated cancer attachment and migration. In addition, morusin reduced the activity of matrix metalloproteinase-2 and 9 (MMP-2 and MMP-9), which were involved in extracellular matrix (ECM) degradation and promoting cancer cell invasion. Furthermore, morusin suppressed the signal transducer and activator of transcription 3 (STAT3) and nuclear factor-kappa B (NF kappa B) signaling pathways, which modulate the protein expression involved in the invasion process. Finally, morusin decreased the lung colonization of the SK-Hep1 cells in the nude mice. These results indicate morusin possesses antitumor progression potential through suppressing STAT3 and NF kappa B.

• 出版日期2015-1-22