Aqueous Extract of Huang-lian Induces Apoptosis in Lung Cancer Cells via P53-Mediated Mitochondrial Apoptosis

作者：Zhu, Deying; Lin, Zhe; Li, Zhenzhong^*

来源：Brazilian Archives of Biology and Technology, 2015, 58(3): 353-357.

DOI：10.1590/S1516-8913201500070

• Huang-lian
• aqueous extract
• apoptosis

The current study was designed to evaluate the activity of the aqueous extract of Huang-lian, and the main apoptosis pathway induced by the extracts of Huang-lian was detected on lung cancer. Antiproliferactive activity was evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and TUNEL methods against human lung cancer cells (A549). Huang-lian regulated the Bcl-2 family protein-mediated mitochondrial pathway via p53 and was detected by western blot. It increased the activation of caspase-3 and caspase-3 cleavage increased as the time increased. These results suggested that Huang-lian regulated the Bcl-2 family protein-mediated mitochondrial via p53 pathway, suggesting that Huang-lian should be further investigation as a natural agent for treating and preventing cancer.

• 出版日期2015-5
• 单位华南理工大学; 深圳华大生命科学研究院