Potential mechanism of mycelium polysaccharide from Pholiota dinghuensis Bi in regulating the proliferation and apoptosis of human breast cancer MCF-7 cells through p38/MAPK pathway

作者：Gan, Dan; Zeng, Xiaoxiong^*; Liu, Rui Hai; Ye, Hong

来源：Journal of Functional Foods, 2015, 12: 375-388.

DOI：10.1016/j.jff.2014.12.008

• Pholiota dinghuensis Bi
• Mycelium polysaccharide
• Cell proliferation
• Apoptosis
• Human breast cancer

The antiproliferative and apoptosis-inducing activities of an acidic polysaccharides fraction from Pholiota dinghuensis Bi mycelium (PDP-3) against human breast cancer MCF-7 cells were investigated. As a result, PDP-3 displayed a significant prevention of cell growth towards MCF-7 cells, with an EC50 value of 38.351 mu g/mL. Furthermore, PDP-3 induced significant apoptosis towards MCF-7 cells. From Western blot results, up-regulated p21 and down-regulated cyclin D1, CDK4 and PCNA, up-regulated Bax and down-regulated Bcl-2, caspase-9 and caspase-3 were found in the protein expressions of MCF-7 cells. In MCF-7 cells treated with PDP-3, down-regulated TRAF2 and up-regulated ASK1, phosphorylated of p38 and p53 were also found. Notably, PDP-3 treatment represented a significant decrease in both nuclear and cytoplasmic expression of ER alpha in MCF-7 cells. Therefore, p38/MAPK signal transduction pathway was the potential mechanism of PDP-3 in regulating both cell proliferation and apoptosis in oestrogen-dependent human breast cancer MCF-7 cells.

• 出版日期2015-1
• 单位南京农业大学