A transitional extracellular matrix instructs cell behavior during muscle regeneration

作者：Calve Sarah; Odelberg Shannon J; Simon Hans Georg^*

来源：Developmental Biology, 2010, 344(1): 259-271.

DOI：10.1016/j.ydbio.2010.05.007

• Extracellular matrix
• Time-lapse microscopy
• 3D imaging
• Skeletal muscle progenitor cells
• Limb regeneration
• Newt

Urodele amphibians regenerate appendages through the recruitment of progenitor cells into a blastema that rebuilds the lost tissue Blastemal formation is accompanied by extensive remodeling of the extracellular matrix Although this remodeling process is important for appendage regeneration, it is not known whether the remodeled matrix directly influences the generation and behavior of blastemal progenitor cells By integrating in vivo 3-dimensional spatiotemporal matrix maps with in vitro functional time-lapse imaging. we show that key components of this dynamic matrix, hyaluronic acid, tenascin-C and fibronectin, differentially direct cellular behaviors including DNA synthesis, migration, myotube fragmentation and myoblast fusion. These data indicate that both satellite cells and fragmenting myofibers contribute to the regeneration blastema and that the local extracellular environment provides instructive cues for the regenerative process. The fact that amphibian and mammali

• 出版日期2010-8-1
• 单位西北大学