科研之友
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摘要
Biodiversity and other branches of biological research require rapid and inexpensive methods to extract and purify DNA, able to process several samples simultaneous ly, thus allowing an extensive approach to the molecular study of diversity in nature. One problem is the necessity of choosing between rapid methods able to produce relatively crude products or time-consuming procedure yielding nucleic acids amenable to all molecular applications, Moreover, the presence of contaminants such as RNA and proteins, absorbing between 230 and 280 nm, hampers the precise quantification of DNA by means of spectrophotometric readings thus forcing to use expensive fluorimetric analyses for reliable measurements. In this work, 70 strains from 29 species and 12 yeast genera, belonging to all major yeast tars, have been employed to set up a method able to extract DNA amenable to spectrophotometric quantification and all molecular applications. The major aim of this procedure is the possibility of extracting pure DNA from all yeast species, including those with a thick capsule, within three days from the inoculation, without the necessity of sophisticated and dedicated equipment.
- 出版日期2001
