A facile and selective approach for enrichment of L-cysteine in human plasma sample based on zinc organic polymer: Optimization by response surface methodology

作者：Bahrani, Sonia; Ghaedi, Mehrorang^*; Ostovan, Abbas; Javadian, Hamedreza; Mansoorkhani, Mohammad Javad Khoshnood; Taghipour, Tahere

来源：Journal of Pharmaceutical and Biomedical Analysis, 2018, 149: 166-171.

DOI：10.1016/j.jpba.2017.10.040

• L-Cysteine
• Enrichment
• Metal organic polymer
• Human blood plasma
• Analysis of variance

In this research, a facile and selective method was described to extract L-cysteine (L-Cys), an essential alpha-amino acid for anti-ageing playing an important role in human health, from human blood plasma sample. The importance of this research was the mild and time-consuming synthesis of zinc organic polymer (Zn-MOP) as an adsorbent and evaluation of its ability for efficient enrichment of L-Cys by ultrasound assisted dispersive micro solid-phase extraction (UA-DMSPE) method. The structure of Zn-MOP was investigated by FT-IR, XRD and SEM. Analysis of variance (ANOVA) was applied for the experimental data to reach the best optimum conditions. The quantification of L-Cys was carried out by high performance liquid chromatography with UV detection set at lambda = 230 nm. The calibration graph showed reasonable linear responses towards L-Cys concentrations in the range of 4.0-1000 mu g/L (r(2) = 0.999) with low limit of detection (0.76 mu g/L, S/N = 3) and RSD <= 2.18 (n = 3). The results revealed the applicability and high performance of this novel strategy in detecting trace L-Cys by Zn-MOP in complicated matrices.

• 出版日期2018-2-5