A NEW FILM FOR RECORDING IMAGES OF SEQUENCING GELS

作者:KRAEV AS*; LI NI; SIDOROV YD; TVERDOKHLEBOV EN
来源:Molecular Biology, 1992, 26(4): 591-593.

摘要

A new film has been developed for the autoradiography and photography of flat objects. It is especially useful for recording electrophoretic patterns in sequencing gels or membranes containing DNA with an isotopic or chemiluminescent label. The film produces sharper images because of the use of a single-sided emulsion. It can be processed at temperatures up to 45-degrees-C and is sufficiently sensitive to produce a sequencing gel pattern in 10 min (chemiluminescent label) or 3 h (P-33 label), starting with about 1 pmole of template DNA.

  • 出版日期1992-8