Application of Electrical Driven and Cationic Exchange Membrane to Capture and Enrich Deoxyribonucleic Acid

作者：Huang Hua Bin; Zhuang Zhi Xia^*; Zhang Wen Hua; Hu Jia; Yang Chao Yong; Wang Xiao Ru

来源：Chinese Journal of Analytical Chemistry, 2010, 38(1): 143-146.

• Deoxyribonucleic acid capture
• Electrical driven
• Ion-exchange membrane
• Concentration

Based on the selective permeability of cationic exchange membrane and the characteristic of electrical driven, a new and non-damage apparatus that can be used to separate compounds with different electric property was built to capture and enrich DNA. A-DNA, as a model compound, was used to study the characteristic of capturing and enriching DNA in this device. Factors that might affect the efficiency such as voltage and flow speed were optimized and gel electrophoresis was done to study if DNA was denaturation, PCR was done to prove the concentration can be used to further bioanalysis. Results were got: the apparatus could used to capture and enrich DNA scathelessly under the condition of low-voltage, the concentrate multiple was 62, and the recovery efficiency was accessible to 47%, concentration can be used for the next bioanalysis. Automatic, easy to handle and little organic reagent using made it be advantageous, it could be miniatured to combine with other biochips. This method had a large application space in field of DNA extraction, purl ion and concentration.

• 出版日期2010-1
• 单位国家海洋局第一海洋研究所; 厦门大学