A major quantitative trait locus conferring subgynoecy in cucumber

作者：Bu, Fengjiao; Chen, Huiming; Shi, Qiuxiang; Zhou, Qian; Gao, Dongli; Zhang, Zhonghua; Huang, Sanwen^*

来源：Theoretical and Applied Genetics, 2016, 129(1): 97-104.

DOI：10.1007/s00122-015-2612-z

• RECOMBINANT INBRED LINES
• SEX EXPRESSION
• SATIVUS L.
• SYNTHASE GENE
• QTL ANALYSIS
• ARABIDOPSIS
• ETHYLENE
• GENOME
• FLOWER
• PLANTS

Cucumber (Cucumis sativus L.) is a model species for sex determination studies, and its yield is associated with the degree of femaleness. Subgynoecy represents a sex form with a high degree of femaleness for which the genetic basis remains elusive. In this study, genetic analysis in the F-2 and BC1 populations developed from a cross between subgynoecious S-2-98 and monoecious M95 suggested a quantitative nature of subgynoecy. Application of simple sequence repeat markers between subgynoecious and monoecious bulks constructed from BC1 plants identified three QTLs: sg3.1, sg6.1, and sg6.2. The major QTL sg3.1 contributed to 54.6 % of the phenotypic variation, and its presence was confirmed by genome-wide comparison of SNP profiles between parental lines and a subgynoecious bulk constructed from BC6 plants. Using PCR-based markers developed from the SNP profile, sg3.1 was further delimited to a genomic region of 799 kb. The genetic basis of subgynoecy revealed here shall shed light on the development of elite cultivars with high yield potential.

• 出版日期2016-1
• 单位中国农业科学院; 湖南省农业科学院