NF135.C10: A New Plasmodium falciparum Clone for Controlled Human Malaria Infections

作者：Teirlinck Anne C; Roestenberg Meta; van de Vegte Bolmer Marga; Scholzen Anja; Heinrichs Moniek J L; Siebelink Stoter Rianne; Graumans Wouter; van Gemert Geert Jan; Teelen Karina; Vos Martijn W; Nganou Makamdop Krystelle; Borrmann Steffen; Rozier Yolanda P A; Erkens Marianne A A; Luty Adrian J F; Hermsen Cornelus C; Sim B Kim Lee; van Lieshout Lisette; Hoffman Stephen L; Visser Leo G; Sauerwein Robert W^*

来源：Journal of Infectious Diseases, 2013, 207(4): 656-660.

DOI：10.1093/infdis/jis725

• Malaria
• parasite culture
• parasite strain
• parasite clone
• field strain
• cellular immunology
• clinical trial
• controlled human malaria infection

We established a new field clone of Plasmodium falciparum for use in controlled human malaria infections and vaccine studies to complement the current small portfolio of P. falciparum strains, primarily based on NF54. The Cambodian clone NF135.C10 consistently produced gametocytes and generated substantial numbers of sporozoites in Anopheles mosquitoes and diverged from NF54 parasites by genetic markers. In a controlled human malaria infection trial, 3 of 5 volunteers challenged by mosquitoes infected with NF135.C10 and 4 of 5 challenged with NF54 developed parasitemia as detected with microscopy. The 2 strains induced similar clinical signs and symptoms as well as cellular immunological responses. Clinical Trials Registration. NCT01002833.

• 出版日期2013-2-15