Deltamethrin increases the fat accumulation in 3T3-L1 adipocytes and Caenorhabditis elegans

作者：Shen Peiyi; Hsieh Tsung Hsiu; Yue Yiren; Sun Quancai; Clark John M; Park Yeonhwa^*

来源：Food and Chemical Toxicology, 2017, 101: 149-156.

DOI：10.1016/j.fct.2017.01.015

• Deltamethrin
• C. elegans
• 3T3-L1
• Fat accumulation
• Obesity

Research has shown that permethrin, a Type-I pyrethroid, increases triglyceride (fat) accumulation in adipocytes. Little is known, however, about any similar effect of deltamethrin, a Type-II pyrethroid, which produces a distinct syndrome of poisoning in marrimals compared with permethrin. This study was therefore aimed to explore the role of deltamethrin on fat accumulation in 3T3-L1 adipocytes and Caenorhabditis elegans. Deltamethrin (10 mu M) significantly increased the fat accumulation in 3T3-L1 adipocytes and wild type C. elegans compared to respective controls. Deltamethtin decreased the ratio of phosphorylated AMP-activated kinase (pAMPK alpha) over AMPK alpha and phosphorylated acetyl-CoA carboxylase (ACC) over ACC, while it increased expression of CCAAT/enhancer-binding protein (C/EBP alpha) and peroxisome proliferator-activated receptor-gamma (PPAR gamma) in 3T3-L1 adipocytes. Similarly, deltamethrin potentiated fat accumulation in C elegans without affecting growth or pharyngeal pumping rate. Moreover, deltamethrin significantly reduced the total progeny number and locomotive activities in C. elegans in a dose-dependent manner. Deltamethrin increased fat accumulation via aak-2 (an ortholog of AMPKa) and nhr-49 (a homolog of peroxisome proliferator-activated receptor-alpha and also downstream target of aak-2) mediated mechanisms. The current work is the first report of the effects of deltamethrin on increased fat storage by 3T3- L1 adipocytes and C. elegans via aak-2 (AMPKa ortholog)-mediated mechanism.

• 出版日期2017-3