A novel regulatory network among LncRpa, CircRar1, MiR-671 and apoptotic genes promotes lead-induced neuronal cell apoptosis

作者：Nan, Aruo; Chen, Lijian; Zhang, Nan; Liu, Zhenzhong; Yang, Ti; Wang, Zhishan; Yang, Chengfeng; Jiang, Yiguo^*

来源：Archives of Toxicology, 2017, 91(4): 1671-1684.

DOI：10.1007/s00204-016-1837-1

• CircRNA
• LncRNA
• MiRNA
• Cell apoptosis
• Lead
• Neurotoxicity

Lead is a metal that has toxic effects on the developing nervous system. However, the mechanisms underlying lead-induced neurotoxicity are not well understood. Non-coding RNAs (ncRNAs) play an important role in epigenetic regulation, but few studies have examined the function of ncRNAs in lead-induced neurotoxicity. We addressed this in the present study by evaluating the functions of a long non-coding RNA (named lncRpa) and a circular RNA (named circRar1) in a mouse model of lead-induced neurotoxicity. High-throughput RNA sequencing showed that both lncRpa and circRar1 promoted neuronal apoptosis. We also found that lncRpa and circRar1 induced the upregulation of apoptosis-associated factors caspase8 and p38 at the mRNA and protein levels via modulation of their common target microRNA miR-671. This is the first report of a regulatory interaction among a lncRNA, circRNA, and miRNA mediating neuronal apoptosis in response to lead toxicity.

• 出版日期2017-4
• 单位广州医科大学; 呼吸疾病国家重点实验室